The pistol, which he is thought to have shot himself with, is in France today, where it is expected to sell for €45,000.
In July 1890, experts believe that the Dutch post-impressionist painter ended his own life with the weapon.
He died in a field near the French town of Auvers-sur-Oise, where the revolver was located some 70 years later.
The artist didn’t die for another 30 hours once wounding himself, before uttering his last words, ‘the sadness will last forever’, according to his brother Theo.
It comes as the world’s most visited multimedia exhibition, displaying Van Gogh’s work, has been touring southern Spain.
Using technology from SENSORY4™, the exhibit allows fans of the Dutch painter to immerse themselves in paintings, such as The Starry Night, on six-metre-high screens.
After passing through Malaga, Sevilla, Alicante and Madrid it will be in Valencia from July 27 to October 20.